Antibody-antigen contacts

Information

Each column shows the extent of antigen contact at each antibody residue for a different antibody (see key below for full pdb codes).

+ indicates an antigen contact
- indicates no antigen contact
. indicates no residue exists in that position in that antibody

Summaries are given for each CDR, where a '+' indicates that at least one residue from the CDR is involved in antigen recognition in that particular antibody.

Non-CDR residue data is shown in parentheses.

Rules governing the use of CDRs and individual residues may be constructed from this data, for example from the CDR summaries:

CDR-L1      +++++-++++-++++++++++++-++
CDR-H1      +-+++++--+++++++++++++++++

we can state that in any antibody EITHER CDR-L1 OR CDR-H1 will make at least one antigen contact.

Note that numbering follows the Chothia scheme.

Methods

CDR definitions and antigen contact criteria are given in the following reference: MacCallum R.M., and Martin A.C.R. and Thornton J.M, (1996), Journal of Molecular Biology, 262 (5), 732-745

The CDR definitions (based on antigen contacts) are also available here.

The data


 (L27 ----------------+---------)

CDR-L1+++++-++++-++++++++++++-++
      aaaaaaaaaaaaaaaaaaaaaaaaaa
      abcdefghijklmnopqrstuvwxyz

  L30 +.------------+----+-.--+-
 L30A +.++.-++++-.-+.+..+.+.--.+
 L30B +.+-..---+-.-+.-..-.-.--.-
 L30C -.--..+----.-+.+..+.+.+-.+
 L30D -.--..-----..-....+.-.--.+
 L30E ..--..+.-.-..+....+.-.+-.-
  L31 ---------------+-+---+--+-
  L32 +++---+-++-+++-++-+++++-++
  L34 -+--+--+-------------+---+
  L36 ----+---------------------

CDR-L2-+--+--+---+--+-++++-+--+-
      aaaaaaaaaaaaaaaaaaaaaaaaaa
      abcdefghijklmnopqrstuvwxyz

  L46 ----+--+------------------
  L49 -+--+--+------+--+-+------
  L50 -+-----+---+----++++-+--+-
  L52 -----------------+--------
  L53 -----------------+-+----+-
  L54 -----------------+--------
  L55 -------+---------+--------
  L56 -----------------+--------

 (L67 ------------------------+-)

CDR-L3++++++++++++++++++++++++++
      aaaaaaaaaaaaaaaaaaaaaaaaaa
      abcdefghijklmnopqrstuvwxyz

  L89 -+--+---------------------
  L91 +++++-+++++++--++++-+++-++
  L92 +-+-----++-+-++-++++-+-++-
  L93 +-++----++-++-+-++-+-+-++-
  L94 ++++-++++++++++++++---+++-
  L96 -+-+++++-++-+++++++-++++++

CDR-H1+-+++++--+++++++++++++++++
      aaaaaaaaaaaaaaaaaaaaaaaaaa
      abcdefghijklmnopqrstuvwxyz

  H30 -------------+--++-+----+-
  H31 --+--------+-++-++++-++-++
 H31B +......-..................
  H32 -----+--------+-++++-+--+-
  H33 --+--++---+++++++++-++++++
  H35 ---+++---+++---++---+-----

CDR-H2++++-+++++++++++++++++++++
      aaaaaaaaaaaaaaaaaaaaaaaaaa
      abcdefghijklmnopqrstuvwxyz

  H47 ---+------+----------++---
  H50 ++-+-++++-++-+--+++-+++-+-
  H51 ----------------------+---
  H52 +-+---++++--+++++++++++++-
 H52A ..+----.+.-++------.--+-.-
  H53 +-+----++----+---+++--+-++
  H54 +-----++-----+++++-+-+--+-
  H55 --------+-------+----++---
  H56 +-----+++-+-++++++--+++-+-
  H57 ---------------------+----
  H58 ------+++---+--++---+++-+-

 (H64 -----------------+--------)

CDR-H3++++++++++++++++++++++++++
      aaaaaaaaaaaaaaaaaaaaaaaaaa
      abcdefghijklmnopqrstuvwxyz

  H93 ----+---------------------
  H95 ---++++++++++++-+-+--++-++
  H96 -+-++--++---++-+++++++---+
  H97 ++++++++++-+-++++++++++--+
  H98 +-+-+-+.----.+++-+-++-+-.+
  H99 -.+---+.----.----+-++-+-.-
 H100 -.++-++.++++.+--.-.-..+-..
H100A +.+----.-+--.---.+....-+..
H100B -.-+-+-.--++.....+....--..
H100C -.....-.-+.+.....-.....-..
H100D .........-.+..............
H100E .........+.+..............
H100F ...........+..............
H100H ...........+..............
 H101 -------+------------------

Key

aa p1acy
ab p1baf
ac p1cbv
ad p1dbb
ae p1eap
af p1fig
ag p1fpt
ah p1ggi
ai p1him
aj p1ibg
ak p1igj
al p1ikf
am p1ind
an p1jel
ao p1jhl
ap p1mfb
aq p1mlc
ar p1ncd
as p1tet
at p1vfb
au p2cgr
av p2hfl
aw p2igf
ax p2mcp
ay p3hfm
az p4fab