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BIOC0016
Mystery Sequence
This web-based tutorial will guide you through using a basic set of
web-based tools to analyze a mystery sequence.
Introduction
This practical will involve you using various protein databases
to obtain as much useful information as you can on a mystery
protein.
This is the type of analysis that will be required for the
coursework, though many other tools are also available.
The objectives of this tutorial are to find out:
- what the protein is
- what it does - its function and what biochemical pathway(s)
it belongs to
- any disease(s) associated with mutations in its
sequence
- the residues implicated in the disease-causing mutants
- the existence of a 3D structure of the protein, or
alternatively of a close relative
- where the disease-causing residues lie in the 3D
structure
- symptoms of any associated disease
- famous sufferers of any associated disease
- a link with the American War of Independence(!)
Instructions
- Use the menu at the top of the page to navigate the sections of
the tutorial. Work along each item in turn, or use the "Continue"
button at the bottom of each page.
- Read all the information on each page! It's
there for a reason!!!
- A link displayed
like this will display a
pop-up with more information when you click on the link.
Warning! You must record information as you go
along where instructed to do so. This will enable you to answer
the questions in the quiz at the end of the tutorial.
When you complete the quiz, you will be asked
to enter your name and email address to confirm that you have
completed the tutorial.
Mystery sequence
Here is the sequence of your mystery protein:
MSGNGNAAATAEENSPKMRVIRVGTRKSQLARIQTDSVVATLKASYPGLQ
FEIIAMSTTGDKILDTALSKIGEKSLFTKELEHALEKNEVDLVVHSLKDL
PTVLPPGFTIGAICKRENPHDAVVFHPKFVGKTLETLPEKSVVGTSSLRR
AAQLQRKFPHLEFRSIRGNLNTRLRKLDEQQEFSAIILATAGLQRMGWHN
RVGQILHPEECMYAVGQGALGVEVRAKDQDILDLVGVLHDPETLLRCIAE
RAFLRHLEGGCSVPVAVHTAMKDGQLYLTGGVWSLDGSDSIQETMQATIH
VPAQHEDGPEDDPQLVGITARNIPRGPQLAAQNLGISLANLLLSKGAKNI
LDVARQLNDAH
This practical was originally written
by Roman
Laskowski.