BIOC0003

Quiz

Congratulations! You have now completed the tutorial

Using the answers that you recorded during the tutorial, now answer the following questions. This is only for your own benefit to ensure you have done all the stages correctly and understand what you are doing. These answers are not recorded.

1. What was the gene map locus for the cystic fibrosis gene?
1q21
7q31.2
7p2.2
10q23.1
19q13.2

2. What is the accession ('entry') code for the human CFTR protein?
P13569
CFTR_HUMAN
P05109
P26361

3. How many amino acids are in the canonical form of the CFTR_HUMAN protein?
168142
13569
1480

4. What is the gene name for the cystic fibrosis transmembrane conductance regulator?
CFTR
Cftr
ATP-binding cassette sub-family C member 7

5. What is the key function of CFTR?
Transport of chloride ions
Transport of potassium ions
Transport of sodium ions

6. How many predicted trans-membrane regions does the CFTR protein have?
2
7
11
12

7. Which of these statements is true?
Humans have fewer enzymes for glycolysis/gluconeogenesis than M. genitalium
Humans have more enzymes for glycolysis/gluconeogenesis than M. genitalium

8. What is the Enzyme Classification number for phosphofructokinase?
3.1.3.11
2.7.1.11
5.3.1.9
4.1.2.13

9. How many different orthologous families of PFK genes are present in E. coli?
1
2
3

10. What is the UniProt accession code for E. coli pfkA?
POA796
P0A796
PO6999
P06999

11. How many amino acids are present in the E. coli protein?
320
963

12. How many bases are present in the E. coli open reading frame (including the stop codon)?
320
963

13. For the supplied prokaryotic DNA sequence, which was the correct 6-frame translation frame?
Forward (5'3') reading frame 1
Forward (5'3') reading frame 2
Forward (5'3') reading frame 3
Reverse (3'5') reading frame 1
Reverse (3'5') reading frame 2
Reverse (3'5') reading frame 3

14. For the FASTA search using the E. coli pfkA protein, other than E. coli, which species has 100% identity?
Buchnera ...
Enterobacter ...
Shigella ...
Photorhabdus ...
Salmonella ...

15. In K6PF1_ECOLI, how many sequence conflict records were there?
1
2
3
4
5

16. What is the function of sequence masking?
To remove 'low complexity' regions or 'simple sequences'
To remove 'high complexity' regions

17. What is the difference between a cladogram and a phylogram?
A cladogram is rooted while a phylogram is unrooted
Both show common ancestry, a phylogram also shows the amount of evolutionary change
Only a phylogram is a phylogenetic tree

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