BIOC0016

Mystery Sequence

This web-based tutorial will guide you through using a basic set of web-based tools to analyze a mystery sequence.

Introduction

This practical will involve you using various protein databases to obtain as much useful information as you can on a mystery protein.

This is the type of analysis that will be required for the coursework, though many other tools are also available.

The objectives of this tutorial are to find out:

what the protein is
what it does - its function and what biochemical pathway(s) it belongs to
any disease(s) associated with mutations in its sequence
the residues implicated in the disease-causing mutants
the existence of a 3D structure of the protein, or alternatively of a close relative
where the disease-causing residues lie in the 3D structure
symptoms of any associated disease
famous sufferers of any associated disease
a link with the American War of Independence(!)

Instructions

Use the menu at the top of the page to navigate the sections of the tutorial. Work along each item in turn, or use the "Continue" button at the bottom of each page.
Read all the information on each page! It's there for a reason!!!
A link displayed like this will display a pop-up with more information when you click on the link.

Warning! You must record information as you go along where instructed to do so. This will enable you to answer the questions in the quiz at the end of the tutorial.

When you complete the quiz, you will be asked to enter your name and email address to confirm that you have completed the tutorial.

Mystery sequence

Here is the sequence of your mystery protein:

MSGNGNAAATAEENSPKMRVIRVGTRKSQLARIQTDSVVATLKASYPGLQ
FEIIAMSTTGDKILDTALSKIGEKSLFTKELEHALEKNEVDLVVHSLKDL
PTVLPPGFTIGAICKRENPHDAVVFHPKFVGKTLETLPEKSVVGTSSLRR
AAQLQRKFPHLEFRSIRGNLNTRLRKLDEQQEFSAIILATAGLQRMGWHN
RVGQILHPEECMYAVGQGALGVEVRAKDQDILDLVGVLHDPETLLRCIAE
RAFLRHLEGGCSVPVAVHTAMKDGQLYLTGGVWSLDGSDSIQETMQATIH
VPAQHEDGPEDDPQLVGITARNIPRGPQLAAQNLGISLANLLLSKGAKNI
LDVARQLNDAH

This practical was originally written by Roman Laskowski.

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