p53 Summary of Mutations and Their Structural Consequences

Contents

Overall Summary

 TotalDistinct
Total mutations 185841827
Complex mutations 7868
Deletions 1661282
Insertions 512200
Silent 1320212
Tandem 14775
Point 152161216
Tandem/point mutations leading to a substitution 136021179
Tandem/point mutations leading to a substitution in the core 13115944

Hydrogen Bonding

We can only calculate these in the core TotalDistinct Total substitutions involving HBonds 6390327 Substitutions involving HBond Acceptors 1990164 Explained sc/sc substitutions involving HBond acceptors)137794 Explained sc/mc substitutions involving HBond acceptors37540 Substitutions involving HBond Donors 5246212 Explained sc/sc substitutions involving HBond donors3836104 Explained sc/mc substitutions involving HBond donors105583


Mutations to Proline

 TotalDistinct
Silent mutations of Proline 11125
Substitutions to Proline 43970
Substitutions to Proline in the Core 41858
Disallowed substitutions to Proline in the Core 39854

Disallowed

CodonAmino acidSecondary StructurePhiPsi
96 ser - - -157.73
110 arg E -144.137 151.752
111 leu E -113.239 147.026
116 ser - -76.39 -39.217
127 ser E -109.949 100.816
130 leu T -85.38 -25.01
136 gln - -100.488 152.672
137 leu T -56.519 131.157
138 ala T 71.046 -6.909
144 gln E -92.668 136.71
145 leu E -100.898 118.692
149 ser S -146.477 133.016
156 arg E -119.194 161.537
158 arg E -127.57 139.746
159 ala E -119.554 146.868
161 ala E -106.536 157.296
165 gln S -71.981 138.63
166 ser T -40.607 -64.889
168 his G -66.524 -13.923
170 thr G -88.444 -18.605
175 arg - -65.109 147.266
178 his H -56.791 -61.784
179 his H -57.75 -31.935
181 arg H -62.976 -43.551
183 ser - -62.278 154.117
192 gln T -84.358 -23.48
193 his - -70.794 133.586
194 leu S -81.987 -44.986
196 arg E -122.688 161.578
200 asn - -141.907 84.965
201 leu T -52.114 2.257
202 arg T -115.442 7.853
213 arg - -37.348 132.738
214 his E -102.983 146.49
228 asp S -113.534 -26.425
230 thr E -120.353 127.212
241 ser T -92.341 12.954
248 arg T 86.753 7.922
252 leu E -118.307 137.257
253 thr E -103.296 127.718
256 thr E -128.545 123.29
257 leu E -83.297 136.58
260 ser T -47.916 -18.882
264 leu E -63.609 126.162
265 leu E -114.011 -21.378
267 arg E -158.106 140.954
271 glu E -89.422 163.063
273 arg E -126.608 110.838
276 ala S -90.697 143.622
280 arg H -64.084 -35.902
282 arg H -80.193 -38.59
283 arg H -50.147 -57.613
284 thr H -52.739 -57.402
289 leu - 90.022 -

Allowed

CodonAmino acidSecondary StructurePhiPsi
140 thr - -66.207 125.858
150 thr - -56.578 129.841
155 thr - -61.553 129.245
189 ala - -63.187 129.726

Mutations from Glycine

 TotalDistinct
Silent mutations of Glycine 8016
Substitutions from Glycine 106378
Substitutions from Glycine in the Core 102761

Disallowed

CodonSubstitutionSecondary StructurePhiPsi
117 arg - 107.595 165.374
117 glu - 107.595 165.374
154 ala T 93.49 -18.147
154 asp T 93.49 -18.147
154 cys T 93.49 -18.147
154 ile T 93.49 -18.147
154 ser T 93.49 -18.147
154 val T 93.49 -18.147
187 arg S 96.541 -9.697
187 asn S 96.541 -9.697
187 asp S 96.541 -9.697
187 cys S 96.541 -9.697
187 ser S 96.541 -9.697
187 val S 96.541 -9.697
244 ala T 97.695 -23.373
244 arg T 97.695 -23.373
244 asp T 97.695 -23.373
244 cys T 97.695 -23.373
244 glu T 97.695 -23.373
244 ser T 97.695 -23.373
244 val T 97.695 -23.373
245 ala T -117.227 -115.488
245 arg T -117.227 -115.488
245 asn T -117.227 -115.488
245 asp T -117.227 -115.488
245 cys T -117.227 -115.488
245 glu T -117.227 -115.488
245 his T -117.227 -115.488
245 leu T -117.227 -115.488
245 phe T -117.227 -115.488
245 ser T -117.227 -115.488
245 val T -117.227 -115.488
262 asp - 106.316 8.547
262 cys - 106.316 8.547
262 his - 106.316 8.547
262 ser - 106.316 8.547
262 val - 106.316 8.547

Allowed

CodonSubstitutionSecondary StructurePhiPsi
105 arg - -101.432 -162.392
105 cys - -101.432 -162.392
105 val - -101.432 -162.392
108 asp - 73.438 59.243
108 ser - 73.438 59.243
112 asp E -122.894 159.618
112 ser E -122.894 159.618
199 ala S 54.791 42.745
199 arg S 54.791 42.745
199 glu S 54.791 42.745
199 val S 54.791 42.745
226 ala T 79.884 2.235
226 asn T 79.884 2.235
226 asp T 79.884 2.235
226 ser T 79.884 2.235
226 val T 79.884 2.235
266 ala E -167.723 162.972
266 arg E -167.723 162.972
266 glu E -167.723 162.972
266 val E -167.723 162.972
279 arg H -68.848 -54.811
279 glu H -68.848 -54.811
279 trp H -68.848 -54.811
279 val H -68.848 -54.811

Structural Clashes

We can only calculate these in the core

 TotalDistinct
Distinct BAD clash mutations- 24
Distinct OK non-clash mutations- 920

DNA Binding

 TotalDistinct
Total residues involved in DNA binding- 14
Number of these actually mutated-14
Substitutions of residues involved in DNA binding 315573

Residues involved

CodonAmino Acid
119 ala
120 lys
121 ser
239 asn
241 ser
243 met
247 asn
248 arg
273 arg
275 cys
276 ala
277 cys
280 arg
283 arg

Zinc Binding

 TotalDistinct
Total residues involved in Zinc binding-4
Number of these actually mutated-4
Total residues involved in Zinc binding 81026

Residues Cys176, His 179, Cys238 and Cys242 are the 4 residues involved in Zinc binding


Cavity-creating Mutations

 TotalDistinct
Number of cavity-creating mutations44526

Mutations Affecting Protein-Interaction Sites

 TotalDistinct
Number of mutations to residues present at protein-interaction sites3033438

Mutations most likely to affect protein-protein or domain-domain interactions (from top 10 scoring patches)

CodonResidueMutation
199 glyglu
166 serleu
166 serthr
174 argtrp
190 proleu
208 aspval
177 proarg
177 proleu
177 proser
202 arghis
211 thrile
158 argcys
158 arggly
158 arghis
158 argleu
158 argpro
168 hisarg
168 histyr
154 glyval
244 glyasp
244 glycys
244 glyser
244 glyval
169 metile
232 ileasn
232 ilethr
142 proleu
243 metile
152 proleu
152 proser
110 argcys
110 argleu
271 glulys
258 gluasp
258 glugln
258 glugly
258 glulys
156 arghis
156 argpro
195 ilephe
195 ilethr
237 metile
132 lysarg
132 lysasn
132 lysgln
132 lysglu
259 asptyr
259 aspval
153 proser
172 valasp
172 valphe
196 argpro
235 asnser
265 leupro
140 thrile

Mutations to Conserved Residues

We can only calculate these in the core

 TotalDistinct
Conserved residues- 63
Mutations to conserved residues 6421349

Conserved Residues

CodonAmino AcidConservation
98 pro 100
113 phe 100
120 lys 100
121 ser 100
122 val 100
125 thr 100
127 ser 100
130 leu 100
132 lys 100
137 leu 100
139 lys 100
142 pro 100
151 pro 100
152 pro 100
158 arg 100
159 ala 100
164 lys 100
172 val 100
173 val 100
175 arg 100
177 pro 100
178 his 100
179 his 100
196 arg 100
198 glu 100
199 gly 100
205 tyr 100
208 asp 100
215 ser 100
216 val 100
218 val 100
219 pro 100
220 tyr 100
221 glu 100
223 pro 100
230 thr 100
239 asn 100
240 ser 100
241 ser 100
242 cys 100
243 met 100
244 gly 100
245 gly 100
247 asn 100
249 arg 100
251 ile 100
253 thr 100
257 leu 100
262 gly 100
265 leu 100
266 gly 100
267 arg 100
270 phe 100
271 glu 100
272 val 100
275 cys 100
276 ala 100
277 cys 100
278 pro 100
279 gly 100
280 arg 100
281 asp 100
282 arg 100


Summary of explained mutants

MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
100arg101       1 39.50
101arg101       1 73.60
101asn101       1 73.60
102ile005         68.70
104his102       1 55.00
104leu101       1 55.00
105arg101       1 16.00
105cys101       1 16.00
105val103       1 16.00
106arg106       1 87.00
106gly101       1 87.00
108asp101       1 9.00
108ser101       1 9.00
109cys111      1  0.40
109leu001         0.40
109ser113      1  0.40
110cys1110  1    1 32.90
110gly111  1    1 32.90
110leu1112  1    1 32.90
110pro116  11   1 32.90
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
110ser111  1    1 32.90
111arg102       1 17.90
111gln104       1 17.90
111pro112   1   1 17.90
112asp101       1 18.60
112ser101       1 18.60
113cys109       1119.80
113gly101       1119.80
113leu101       1119.80
113ser103       1119.80
113val105       1119.80
115tyr102       1 98.90
116cys112  1    1 40.70
116pro111  11   1 40.70
117arg112    1    40.60
117glu113    1    40.60
119thr1111        60.40
120arg1121       160.60
120met1111       160.60
121phe1111 1     139.20
122leu101        119.60
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
123asn001         39.70
123ile001         39.70
124arg103       1 8.70
124ser101       1 8.70
124trp101       1 8.70
125arg111  1     10.60
125lys113  1     10.60
125met117  1     10.60
126asn113  1      17.00
126asp114  1      17.00
126cys117  1      17.00
126gly111  1      17.00
126his112  1      17.00
126phe111  1      17.00
126ser111  1      17.00
127cys111  1     10.00
127phe1116  1  1  10.00
127pro115  11    10.00
127thr102        10.00
127tyr114     1  10.00
128ala001         72.00
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
128arg001         72.00
128leu003         72.00
128ser008         72.00
129asp003         87.60
129thr003         87.60
129val003         87.60
130arg109        127.70
130his101        127.70
130ile101        127.70
130phe1010        127.70
130pro112   1    127.70
130val1015        127.70
131asp001         42.60
131his113  1      42.60
131ile115  1      42.60
131lys002         42.60
131ser003         42.60
131tyr113  1      42.60
132arg1041       116.10
132asn1034       116.10
132gln1014       116.10
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
132glu1119  1    116.10
132leu111  1    116.10
132met119  1    116.10
132thr103       116.10
132trp101       116.10
133arg003         0.10
133ile006         0.10
133leu003         0.10
133lys009         0.10
133thr114      1  0.10
133val112      1  0.10
134cys005         0.00
134ile002         0.00
134leu0017         0.00
134ser001         0.00
134val003         0.00
135arg118  1  1   0.00
135gly117  1      0.00
135phe1135  1      0.00
135ser1112  1      0.00
135trp1121  1  1   0.00
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
135tyr1157  1  1   0.00
136arg006         17.80
136glu005         17.80
136his003         17.80
136lys001         17.80
136pro112   1     17.80
137gln103        127.40
137met103        127.40
137pro113   1    127.40
137val103        127.40
138asp003         45.60
138pro1122   1     45.60
138thr005         45.60
138val0038         45.60
139arg103        144.00
139asn1010        144.00
139gln102        144.00
139glu102        144.00
139thr101        144.00
140ala114  1    1 30.70
140ile1111  1    1 30.70
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
140pro111  1    1 30.70
140ser102       1 30.70
141arg1115  1      0.00
141gly111  1      0.00
141phe115  1      0.00
141ser113  1      0.00
141trp1111  1      0.00
141tyr1167  1      0.00
142ala102       1116.40
142his101       1116.40
142leu1012       1116.40
142phe103       1116.40
142ser106       1116.40
142thr101       1116.40
143ala1116      1  0.00
143glu004         0.00
143gly111      1  0.00
143leu003         0.00
143met0020         0.00
144arg117  1    1 18.80
144his115  1    1 18.80
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
144leu115  1    1 18.80
144lys111  1    1 18.80
144pro117  11   1 18.80
145arg003         0.70
145gln0012         0.70
145met002         0.70
145pro1113   1  1  0.70
145val004         0.70
146arg106       1 33.10
146cys111  1    1 33.10
146gly111  1    1 33.10
146leu112  1    1 33.10
146ser103       1 33.10
147ala104       1 16.90
147asp105       1 16.90
147glu104       1 16.90
147gly106       1 16.90
147ile106       1 16.90
148ala101       1 75.00
148asn104       1 75.00
148glu104       1 75.00
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
148tyr103       1 75.00
148val101       1 75.00
149phe105       1 45.00
149pro114   1   1 45.00
149thr103       1 45.00
150ala101       1 60.30
150arg101       1 60.30
150ile106       1 60.30
150lys103       1 60.30
150pro101       1 60.30
151ala119      1 13.10
151arg1011        13.10
151his1028        13.10
151leu1012        13.10
151ser1172      1 13.10
151thr1015        13.10
152ala102       1143.90
152arg103       1143.90
152gln104       1143.90
152leu1057       1143.90
152ser1023       1143.90
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
152thr106       1143.90
153ala102       1 93.00
153his101       1 93.00
153leu105       1 93.00
153ser1010       1 93.00
153thr104       1 93.00
154ala111    1  1 45.00
154asp117    1  1 45.00
154cys111    1  1 45.00
154ile111    1  1 45.00
154ser119    1  1 45.00
154val1144    1  1 45.00
155ala118  1      2.50
155asn1114  1      2.50
155ile1113  1      2.50
155met111  1      2.50
155pro1114  1      2.50
155ser115  1      2.50
156cys107       1 23.20
156gly104       1 23.20
156his1014       1 23.20
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
156leu101       1 23.20
156pro1131   1   1 23.20
156ser104       1 23.20
157ala001         0.70
157asp009         0.70
157gly0011         0.70
157ile0011         0.70
157leu009         0.70
157phe00126         0.70
158cys1111  1    1114.00
158gln101       1114.00
158gly1120  1    1114.00
158his1168  1    1114.00
158leu1173  1    1114.00
158phe111  1    1114.00
158pro1113  11   1114.00
158ser102       1114.00
158tyr101       1114.00
159asp1011        10.20
159gly102        10.20
159pro1122   1    10.20
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
159ser103        10.20
159thr108        10.20
159val1037        10.20
160ile109       1 14.10
160leu105       1 14.10
160lys103       1 14.10
160val108       1 14.10
161asp1011       1 8.80
161gly104       1 8.80
161phe101       1 8.80
161pro112   1   1 8.80
161ser101       1 8.80
161thr1052       1 8.80
161val1010       1 8.80
162asn002         0.20
162met006         0.20
162phe007         0.20
162ser007         0.20
162thr003         0.20
162val005         0.20
163asn0021         1.00
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
163asp003         1.00
163cys0099         1.00
163his0016         1.00
163ser003         1.00
164arg102        146.80
164asn108        146.80
164gln105        146.80
164glu1015        146.80
164met104        146.80
164thr102        146.80
165arg004         38.10
165glu002         38.10
165his001         38.10
165leu004         38.10
165pro113   1     38.10
166ala103       1 76.90
166gly101       1 76.90
166leu1010       1 76.90
166pro112   1   1 76.90
166thr1013       1 76.90
167arg119  1    1 67.10
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
167his119  1    1 67.10
167leu112  1    1 67.10
167lys111  1    1 67.10
168arg1021       1 28.30
168asn102       1 28.30
168asp102       1 28.30
168gln102       1 28.30
168leu105       1 28.30
168pro116   1   1 28.30
168tyr1010       1 28.30
168val101       1 28.30
169ile1014       1 21.20
169lys102       1 21.20
169thr104       1 21.20
169val106       1 21.20
170ala114  1    1 65.90
170lys101       1 65.90
170met117  1    1 65.90
170pro111  11   1 65.90
170ser112  1    1 65.90
171ala111  1    1 45.30
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
171asp102       1 45.30
171gln101       1 45.30
171gly115  1    1 45.30
171lys118  1    1 45.30
171val111  1    1 45.30
172ala109       1124.50
172asp1010       1124.50
172gly105       1124.50
172ile104       1124.50
172phe1012       1124.50
173ala1015       116.10
173glu102       116.10
173gly1113      1116.10
173leu1071       116.10
173met1052       116.10
173trp101       116.10
174gly101       1 40.80
174lys108       1 40.80
174met102       1 40.80
174ser107       1 40.80
174thr101       1 40.80
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
174trp1012       1 40.80
175cys1119  1     16.50
175gln101        16.50
175gly1119  1     16.50
175his11828  1     16.50
175leu1122  1     16.50
175pro115  11    16.50
175ser1110  1     16.50
176arg1110 11      4.10
176gly116 11      4.10
176phe11143 11      4.10
176ser1125 11      4.10
176trp1112 11      4.10
176tyr1159 11      4.10
177ala101       1164.10
177arg1012       1164.10
177his103       1164.10
177ile101       1164.10
177leu1027       1164.10
177phe103       1164.10
177ser1012       1164.10
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
178arg102        160.50
178asn107        160.50
178asp109        160.50
178gln101        160.50
178leu101        160.50
178pro117   1    160.50
178tyr109        160.50
179arg11113 11     127.50
179asn1114 11     127.50
179asp1118 11     127.50
179gln1115 11     127.50
179leu1130 11     127.50
179pro113 111    127.50
179tyr1173 11     127.50
180ala001         30.90
180asp001         30.90
180gln001         30.90
180gly001         30.90
180lys006         30.90
180val001         30.90
181cys0020         82.30
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
181gly002         82.30
181his0026         82.30
181leu004         82.30
181pro1119   1     82.30
181ser002         82.30
182arg003         54.80
182ser006         54.80
182tyr004         54.80
183leu112  1      27.70
183pro113  11     27.70
184asn0011         63.30
184gly111  1      63.30
184his114  1      63.30
184tyr003         63.30
185arg112  1      52.70
185asn111  1      52.70
185cys111  1      52.70
185gly112  1      52.70
185ile112  1      52.70
186asn112  1      55.50
186glu111  1      55.50
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
186gly112  1      55.50
186his112  1      55.50
186tyr111  1      55.50
186val112  1      55.50
187arg112    1    95.20
187asn111    1    95.20
187asp113    1    95.20
187cys115    1    95.20
187ser117    1    95.20
187val112    1    95.20
188val004         42.30
189asp001         3.60
189gly002         3.60
189pro004         3.60
189thr005         3.60
189val0012         3.60
190ala101       1 23.00
190arg103       1 23.00
190his101       1 23.00
190leu1026       1 23.00
190ser103       1 23.00
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
190thr103       1 23.00
191arg101       1 43.90
191his103       1 43.90
191leu104       1 43.90
191ser103       1 43.90
191thr104       1 43.90
192arg108       1 21.10
192his115  1    1 21.10
192leu113  1    1 21.10
192lys101       1 21.10
192pro111  11   1 21.10
193arg0052         1.90
193asn003         1.90
193asp0010         1.90
193gln002         1.90
193leu0038         1.90
193pro1111   1     1.90
193tyr0028         1.90
194arg0039         3.10
194his005         3.10
194ile114      1  3.10
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
194phe0019         3.10
194pro1112   1  1  3.10
194val002         3.10
195asn106       1 5.30
195phe1020       1 5.30
195ser105       1 5.30
195thr1056       1 5.30
195tyr101       1 5.30
195val101       1 5.30
196gln116  1    115.40
196leu112  1    115.40
196pro1114  11   115.40
196ser111  1    115.40
197glu005         3.90
197gly0010         3.90
197leu003         3.90
197met005         3.90
198asp101       1140.50
198gln102       1140.50
198gly111  1    1140.50
198lys104       1140.50
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
199ala101       11104.50
199arg106       11104.50
199glu1011       11104.50
199val103       11104.50
200asp101       1 32.20
200lys111  1    1 32.20
200pro111  11   1 32.20
200ser102       1 32.20
201phe108       1 92.20
201pro111   1   1 92.20
202cys104       1 70.60
202gly101       1 70.60
202his1010       1 70.60
202leu105       1 70.60
202pro115   1   1 70.60
202ser106       1 70.60
203ala102       1 7.80
203glu109       1 7.80
203leu106       1 7.80
203met101       1 7.80
203trp111     1 1 7.80
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
204ala101       1 43.50
204gly101       1 43.50
204lys103       1 43.50
204val101       1 43.50
205asn112  1    1116.20
205asp1111  1    1116.20
205cys1186  1   11116.20
205his116  1    1116.20
205phe114  1    1116.20
205ser1112  1   11116.20
206met101       1 32.80
206phe101       1 32.80
207asn111  1    1 35.40
207glu112  1    1 35.40
207gly113  1    1 35.40
207his112  1    1 35.40
207val111  1    1 35.40
208asn106       1136.60
208glu102       1136.60
208gly104       1136.60
208his101       1136.60
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
208tyr101       1136.60
208val1012       1136.60
209ile101       1 88.20
209lys105       1 88.20
209thr103       1 88.20
210asp103       1 87.10
210his103       1 87.10
210ile101       1 87.10
210ser103       1 87.10
211ala104       1 24.70
211asn103       1 24.70
211ile1013       1 24.70
212leu107       1 69.00
212ser103       1 69.00
212tyr101       1 69.00
212val101       1 69.00
213gln1130  1      3.60
213gly115  1      3.60
213leu1124  1      3.60
213pro113  11     3.60
214arg1051       1 9.60
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
214asp113  1    1 9.60
214gln104       1 9.60
214pro111  11   1 9.60
214tyr105       1 9.60
215arg1111  1     12.40
215asn1012        12.40
215cys114  1     12.40
215gly117  1     12.40
215ile1112  1     12.40
215lys112  1     12.40
215thr102        12.40
216ala104        11.30
216glu104        11.30
216gly113      1 11.30
216leu1012        11.30
216met1045        11.30
216trp101        11.30
217ala003         1.50
217glu004         1.50
217gly003         1.50
217leu002         1.50
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
217met003         1.50
218ala104        11.70
218glu107        11.70
218gly108        11.70
218leu103        11.70
218met1015        11.70
219cys101       1120.50
219his101       1120.50
219leu107       1120.50
219ser104       1120.50
219thr102       1120.50
220asn118      1118.00
220asp102       118.00
220cys10233       118.00
220his1011       118.00
220ser1010       118.00
221ala103       1168.30
221asp105       1168.30
221gln102       1168.30
221gly102       1168.30
221lys103       1168.30
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
222arg101       1 54.70
222gln104       1 54.70
222leu105       1 54.70
222ser102       1 54.70
222thr101       1 54.70
223ala101       1128.10
223his104       1128.10
223leu102       1128.10
223ser101       1128.10
224asp108       1 82.10
224gly102       1 82.10
224lys106       1 82.10
224val102       1 82.10
225ala102       1 103.80
225asp101       1 103.80
225gly101       1 103.80
225ile101       1 103.80
225leu101       1 103.80
225phe102       1 103.80
226ala103       1 104.90
226asn101       1 104.90
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
226asp103       1 104.90
226ser101       1 104.90
226val101       1 104.90
227cys101       1 49.40
227phe107       1 49.40
227thr101       1 49.40
228ala102       1 77.30
228asn106       1 77.30
228glu105       1 77.30
228gly106       1 77.30
228his102       1 77.30
228pro111   1   1 77.30
228tyr101       1 77.30
228val101       1 77.30
229arg102       1 21.60
229asn101       1 21.60
229gly101       1 21.60
229ser105       1 21.60
229tyr103       1 21.60
230ala113  1    1111.70
230asn103       1111.70
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
230ile118  1    1111.70
230pro112  11   1111.70
230ser102       1111.70
231ala103       1 40.60
231asn102       1 40.60
231ile103       1 40.60
231ser103       1 40.60
232asn1010       1 6.40
232leu102       1 6.40
232phe106       1 6.40
232ser107       1 6.40
232thr1010       1 6.40
232val104       1 6.40
233arg101       1 40.00
233asp101       1 40.00
233gln103       1 40.00
233leu101       1 40.00
233tyr104       1 40.00
234asn0010         1.10
234asp113      1  1.10
234cys1183      1  1.10
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
234his0015         1.10
234phe001         1.10
234ser116      1  1.10
235asp116  1    1 11.00
235his111  1    1 11.00
235ile114  1    1 11.00
235ser1113  1    1 11.00
235thr114  1    1 11.00
235tyr114     1 1 11.00
236asn1115  1      0.00
236asp116  1      0.00
236cys1145  1   1  0.00
236his118  1      0.00
236phe111  1      0.00
236ser113  1   1  0.00
237arg106       1 7.70
237ile10132       1 7.70
237leu105       1 7.70
237lys106       1 7.70
237thr103       1 7.70
237val108       1 7.70
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
238arg1118 11      0.50
238gly119 11      0.50
238his111 11      0.50
238phe1129 11  1   0.50
238ser1119 11      0.50
238trp115 11  1   0.50
238tyr1158 11  1   0.50
239asp11311       130.60
239his1111       130.60
239ile1111       130.60
239lys1191       130.60
239ser11271       130.60
239thr1191       130.60
239tyr1181       130.60
240arg1111  1     18.70
240asn114  1     18.70
240cys114  1     18.70
240gly1115  1     18.70
240ile116  1     18.70
240thr115  1     18.70
241ala1191       140.10
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
241cys11241       140.10
241phe11831       140.10
241pro1181  1    140.10
241thr1171       140.10
241tyr11111       140.10
242arg118 11     110.10
242gly115 11     110.10
242phe1157 11  1  110.10
242ser1128 11     110.10
242trp1113 11  1  110.10
242tyr1139 11  1  110.10
243arg1121      1161.20
243ile11101      1161.20
243leu1161      1161.20
243lys1131      1161.20
243thr1161      1161.20
243val1121      1161.20
244ala114    1  1169.10
244arg114    1  1169.10
244asp1142    1  1169.10
244cys1136    1  1169.10
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
244glu111    1  1169.10
244ser1147    1  1169.10
244val1114    1  1169.10
245ala118    1   17.50
245arg1113    11  17.50
245asn113    1   17.50
245asp11105    1   17.50
245cys1168    1   17.50
245glu111    1   17.50
245his111    11  17.50
245leu119    1   17.50
245phe111    11  17.50
245ser11296    1   17.50
245val1156    1   17.50
246arg0013         0.50
246ile0035         0.50
246leu007         0.50
246lys007         0.50
246thr0010         0.50
246val0038         0.50
247asp1161      1147.30
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
247ile1171      1147.30
247lys1131      1147.30
247ser1121      1147.30
247thr1171      1147.30
247tyr1111      1147.30
248gln116131        70.90
248gly11171        70.90
248leu11781        70.90
248pro11131  1     70.90
248trp114991        70.90
249asn111  1     114.20
249gly1129  1     114.20
249ile111  1     114.20
249lys1116  1     114.20
249met1152  1     114.20
249ser11311  1     114.20
249thr1119  1     114.20
249trp1125  1     114.20
250ala0011         32.50
250asn002         32.50
250gln002         32.50
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
250his002         32.50
250leu0036         32.50
250phe002         32.50
250ser0014         32.50
250thr005         32.50
251asn108        10.10
251leu103        10.10
251phe105        10.10
251ser1022        10.10
251thr104        10.10
251val105        10.10
252his001         7.80
252phe004         7.80
252pro116   1     7.80
252val001         7.80
253ala114  1     10.00
253asn102        10.00
253ile117  1     10.00
253pro113  11    10.00
253ser104        10.00
254asn0012         5.00
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
254asp003         5.00
254leu001         5.00
254met001         5.00
254phe001         5.00
254ser005         5.00
254thr006         5.00
254val004         5.00
255asn005         0.00
255met002         0.00
255phe0029         0.00
255ser006         0.00
255thr009         0.00
255val002         0.00
256ala106       1 13.00
256arg101       1 13.00
256ile103       1 13.00
256lys103       1 13.00
256pro111   1   1 13.00
256ser102       1 13.00
257arg102        10.20
257gln1112      1 10.20
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
257pro119   1  1 10.20
257val115      1 10.20
258ala111  1    1 6.80
258asp1011       1 6.80
258gln1013       1 6.80
258gly1114  1    1 6.80
258leu111  1    1 6.80
258lys1150  1    1 6.80
258val112  1    1 6.80
259asn105       1 12.90
259glu104       1 12.90
259gly114  1    1 12.90
259his113  1    1 12.90
259pro100       1 12.90
259tyr1114  1    1 12.90
259val1116  1    1 12.90
260ala113  1    1 71.80
260cys111  1    1 71.80
260phe113  1    1 71.80
260pro112  11   1 71.80
260thr101       1 71.80
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
260tyr111  1    1 71.80
261arg104       1 69.60
261asn102       1 69.60
261cys111  1    1 69.60
261gly112  1    1 69.60
261ile112  1    1 69.60
262asp112    1  1146.90
262cys111    1  1146.90
262his111    1  1146.90
262ser115    1  1146.90
262val116    1  1146.90
263asp103       1 66.10
263his101       1 66.10
263ile102       1 66.10
263ser101       1 66.10
264gln101       1 42.70
264ile103       1 42.70
264pro111   1   1 42.70
265arg103       1116.20
265gln104       1116.20
265met103       1116.20
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
265pro1115   1   1116.20
266ala101        10.10
266arg1151     1  10.10
266glu1048        10.10
266val1029        10.10
267gln109        112.90
267gly113  1     112.90
267his111  1     112.90
267pro1115  11    112.90
267trp1121  1     112.90
268his113  1      15.80
268ile111  1      15.80
268ser118  1      15.80
268tyr111  1      15.80
269arg001         23.70
269asn008         23.70
269cys002         23.70
269gly003         23.70
269ile001         23.70
269thr001         23.70
270cys1020        10.70
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
270ile1013        10.70
270leu1022        10.70
270ser1113      1 10.70
270tyr105        10.70
270val117      1 10.70
271ala101       1111.20
271arg101       1111.20
271asp102       1111.20
271gln105       1111.20
271gly103       1111.20
271lys1025       1111.20
271pro111   1   1111.20
271val104       1111.20
272ala107        11.10
272glu108        11.10
272gly105        11.10
272leu1034        11.10
272met1064        11.10
273asn1111        33.60
273cys114681 1      33.60
273gln1111        33.60
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
273gly11101 1      33.60
273his115441 1      33.60
273leu111081 1      33.60
273pro11261 11     33.60
273ser11111 1      33.60
273tyr1111        33.60
274ala0013         1.00
274asp006         1.00
274gly005         1.00
274ile004         1.00
274leu0012         1.00
274phe0020         1.00
275arg11121 1     17.90
275gly1161 1     17.90
275phe11281 1     17.90
275ser1131       17.90
275trp1161 1     17.90
275tyr11521       17.90
276asp1191       162.60
276gly1141       162.60
276pro11151  1    162.60
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
276ser11191       162.60
276thr11101       162.60
276val1171       162.60
277arg1111       114.10
277gly1171 1     114.10
277phe11351 1     114.10
277ser1111       114.10
277trp1111 1     114.10
277tyr11241 1     114.10
278ala1015        10.00
278arg1129     1  10.00
278his1110     1  10.00
278leu1060        10.00
278phe118     1  10.00
278ser1058        10.00
278thr1025        10.00
279arg118     1  10.00
279glu1133     1  10.00
279trp114     1  10.00
279val112     1  10.00
280gly11281 1     147.80
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
280ile11151 1     147.80
280lys11511       147.80
280pro1111 11    147.80
280ser11131       147.80
280thr11671       147.80
281ala112  1     112.00
281asn1124  1     112.00
281glu1139  1     112.00
281gly1113  1     112.00
281his1129  1     112.00
281tyr117  1     112.00
281val114  1     112.00
282gln1022        113.60
282gly1135  1     113.60
282his111  1     113.60
282leu118  1     113.60
282pro1112  11    113.60
282trp10399        113.60
283cys11181 1      45.70
283gly1141 1      45.70
283his1191 1      45.70
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
283leu1151 1      45.70
283pro11251 11     45.70
283ser1111        45.70
284ala113  1      42.40
284ile112  1      42.40
284lys112  1      42.40
284pro115  11     42.40
285ala111  1      26.60
285asp001         26.60
285gln006         26.60
285gly112  1      26.60
285lys11120  1      26.60
285val1114  1      26.60
286ala112  1      32.10
286asp001         32.10
286gln0011         32.10
286gly1117  1      32.10
286leu111  1      32.10
286lys0061         32.10
286val115  1      32.10
287asp004         67.80
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
287gly002         67.80
287lys008         67.80
287val002         67.80
288asp001         67.20
288lys001         67.20
288ser005         67.20
288thr002         67.20
288tyr005         67.20
289arg001         110.40
289his001         110.40
289phe0010         110.40
289pro113   1     110.40
289val001         110.40
94leu002         0.00
94thr001         0.00
95thr001         0.00
96cys101       1 88.80
96phe101       1 88.80
96pro111   1   1 88.80
97phe101       1 48.40
98leu101       1126.90
MutationExplained?Struc-Explained?NExamplesDNAZincHBondProGlyClashCavitiesIn PatchConservedAccessibility
98ser101       1126.90
82.4342.227.722.7526.885.713.922.542.7546.3536.93

Explained 779 mutants out of 945 distinct (82.433862%)
Structurally explained 399 mutants out of 945 distinct (42.222222%)
Mutations explained in structural terms and by patch analysis: 702 out of 945 distinct (74.285714%)
Mutations explained by patch analysis only: 438 out of 945 distinct (46.349206%)
Explained 11710 mutants out of 13115 observed (89.287076%)