INPUT DETAILS
Number of Residues:260
Sequence ID:sp|P17261|ERS1_YEAST Transmembrane protein ERS1 (ERD suppressor) - Saccharomyces cerevisiae (Baker's yeast).
Sequence:
MVSLDDILGIVYVTSWSISMYPPIITNWRHKSASAISMDFVMLNTAGYSYLVISIFLQLY
CWKMTGDESDLGRPKLTQFDFWYCLHGCLMNVVLLTQVVAGARIWRFPGKGHRKMNPWYL
RILLASLAIFSLLTVQFMYSNYWYDWHNSRTLAYCNNLFLLKISMSLIKYIPQVTHNSTR
KSMDCFPIQGVFLDVTGGIASLLQLIWQLSNDQGFSLDTFVTNFGKVGLSMVTLIFNFIF
IMQWFVYRSRGHDLASEYPL
Email address:s.v.v.deevi@rdg.ac.uk
Parameters:
Server : targetp Parameter Name : origin Value : non-plant
Server : psort Parameter Name : origin Value : yeast
Analysis results from program: NetPhos
Running NetPhos Version 2.0
Function : Protein Phosphorylation sites Prediction
| Residue | M | V | S | L | D | D | I | L | G | I | V | Y | V | T | S |
| Number | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 |
| NetPhos | P-score | 0.000 | 0.000 | 0.033 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.041 | 0.000 | 0.022 | 0.063 |
| Residue | W | S | I | S | M | Y | P | P | I | I | T | N | W | R | H |
| Number | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 |
| NetPhos | P-score | 0.000 | 0.148 | 0.000 | 0.799 | 0.000 | 0.015 | 0.000 | 0.000 | 0.000 | 0.000 | 0.049 | 0.000 | 0.000 | 0.000 | 0.000 |
| Residue | K | S | A | S | A | I | S | M | D | F | V | M | L | N | T |
| Number | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 |
| NetPhos | P-score | 0.000 | 0.295 | 0.000 | 0.973 | 0.000 | 0.000 | 0.126 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.024 |
| Residue | A | G | Y | S | Y | L | V | I | S | I | F | L | Q | L | Y |
| Number | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 |
| NetPhos | P-score | 0.000 | 0.000 | 0.081 | 0.007 | 0.061 | 0.000 | 0.000 | 0.000 | 0.012 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.023 |
| Residue | C | W | K | M | T | G | D | E | S | D | L | G | R | P | K |
| Number | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 |
| NetPhos | P-score | 0.000 | 0.000 | 0.000 | 0.000 | 0.717 | 0.000 | 0.000 | 0.000 | 0.345 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| Residue | L | T | Q | F | D | F | W | Y | C | L | H | G | C | L | M |
| Number | 76 | 77 | 78 | 79 | 80 | 81 | 82 | 83 | 84 | 85 | 86 | 87 | 88 | 89 | 90 |
| NetPhos | P-score | 0.000 | 0.866 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.282 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| Residue | N | V | V | L | L | T | Q | V | V | A | G | A | R | I | W |
| Number | 91 | 92 | 93 | 94 | 95 | 96 | 97 | 98 | 99 | 100 | 101 | 102 | 103 | 104 | 105 |
| NetPhos | P-score | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.330 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| Residue | R | F | P | G | K | G | H | R | K | M | N | P | W | Y | L |
| Number | 106 | 107 | 108 | 109 | 110 | 111 | 112 | 113 | 114 | 115 | 116 | 117 | 118 | 119 | 120 |
| NetPhos | P-score | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.513 | 0.000 |
| Residue | R | I | L | L | A | S | L | A | I | F | S | L | L | T | V |
| Number | 121 | 122 | 123 | 124 | 125 | 126 | 127 | 128 | 129 | 130 | 131 | 132 | 133 | 134 | 135 |
| NetPhos | P-score | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.005 | 0.000 | 0.000 | 0.000 | 0.000 | 0.002 | 0.000 | 0.000 | 0.005 | 0.000 |
| Residue | Q | F | M | Y | S | N | Y | W | Y | D | W | H | N | S | R |
| Number | 136 | 137 | 138 | 139 | 140 | 141 | 142 | 143 | 144 | 145 | 146 | 147 | 148 | 149 | 150 |
| NetPhos | P-score | 0.000 | 0.000 | 0.000 | 0.048 | 0.042 | 0.000 | 0.011 | 0.000 | 0.545 | 0.000 | 0.000 | 0.000 | 0.000 | 0.007 | 0.000 |
| Residue | T | L | A | Y | C | N | N | L | F | L | L | K | I | S | M |
| Number | 151 | 152 | 153 | 154 | 155 | 156 | 157 | 158 | 159 | 160 | 161 | 162 | 163 | 164 | 165 |
| NetPhos | P-score | 0.006 | 0.000 | 0.000 | 0.641 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.075 | 0.000 |
| Residue | S | L | I | K | Y | I | P | Q | V | T | H | N | S | T | R |
| Number | 166 | 167 | 168 | 169 | 170 | 171 | 172 | 173 | 174 | 175 | 176 | 177 | 178 | 179 | 180 |
| NetPhos | P-score | 0.050 | 0.000 | 0.000 | 0.000 | 0.040 | 0.000 | 0.000 | 0.000 | 0.000 | 0.295 | 0.000 | 0.000 | 0.216 | 0.046 | 0.000 |
| Residue | K | S | M | D | C | F | P | I | Q | G | V | F | L | D | V |
| Number | 181 | 182 | 183 | 184 | 185 | 186 | 187 | 188 | 189 | 190 | 191 | 192 | 193 | 194 | 195 |
| NetPhos | P-score | 0.000 | 0.985 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| Residue | T | G | G | I | A | S | L | L | Q | L | I | W | Q | L | S |
| Number | 196 | 197 | 198 | 199 | 200 | 201 | 202 | 203 | 204 | 205 | 206 | 207 | 208 | 209 | 210 |
| NetPhos | P-score | 0.013 | 0.000 | 0.000 | 0.000 | 0.000 | 0.006 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.011 |
| Residue | N | D | Q | G | F | S | L | D | T | F | V | T | N | F | G |
| Number | 211 | 212 | 213 | 214 | 215 | 216 | 217 | 218 | 219 | 220 | 221 | 222 | 223 | 224 | 225 |
| NetPhos | P-score | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.011 | 0.000 | 0.000 | 0.034 | 0.000 | 0.000 | 0.121 | 0.000 | 0.000 | 0.000 |
| Residue | K | V | G | L | S | M | V | T | L | I | F | N | F | I | F |
| Number | 226 | 227 | 228 | 229 | 230 | 231 | 232 | 233 | 234 | 235 | 236 | 237 | 238 | 239 | 240 |
| NetPhos | P-score | 0.000 | 0.000 | 0.000 | 0.000 | 0.205 | 0.000 | 0.000 | 0.006 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| Residue | I | M | Q | W | F | V | Y | R | S | R | G | H | D | L | A |
| Number | 241 | 242 | 243 | 244 | 245 | 246 | 247 | 248 | 249 | 250 | 251 | 252 | 253 | 254 | 255 |
| NetPhos | P-score | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.050 | 0.000 | 0.013 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| Residue | S | E | Y | P | L |
| Number | 256 | 257 | 258 | 259 | 260 |
| NetPhos | P-score | 0.300 | 0.000 | 0.017 | 0.000 | 0.000 |
Threshold description :
P-scores greater than 0.5 are considered as positive predictions
Graphical Output :
Analysis results from program: NetOGlyc
Running NetOGlyc Version 3.1
Function : Protein Glycosylation sites Prediction
| Residue | M | V | S | L | D | D | I | L | G | I | V | Y | V | T | S |
| Number | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 |
| NetOGlyc | gscore | 0.000 | 0.000 | 0.312 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.256 | 0.174 |
| iscore | 0.000 | 0.000 | 0.055 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.070 | 0.051 |
| Residue | W | S | I | S | M | Y | P | P | I | I | T | N | W | R | H |
| Number | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 |
| NetOGlyc | gscore | 0.000 | 0.179 | 0.000 | 0.185 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.377 | 0.000 | 0.000 | 0.000 | 0.000 |
| iscore | 0.000 | 0.050 | 0.000 | 0.029 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.077 | 0.000 | 0.000 | 0.000 | 0.000 |
| Residue | K | S | A | S | A | I | S | M | D | F | V | M | L | N | T |
| Number | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 |
| NetOGlyc | gscore | 0.000 | 0.226 | 0.000 | 0.221 | 0.000 | 0.000 | 0.204 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.151 |
| iscore | 0.000 | 0.027 | 0.000 | 0.026 | 0.000 | 0.000 | 0.057 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.052 |
| Residue | A | G | Y | S | Y | L | V | I | S | I | F | L | Q | L | Y |
| Number | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 |
| NetOGlyc | gscore | 0.000 | 0.000 | 0.000 | 0.074 | 0.000 | 0.000 | 0.000 | 0.000 | 0.083 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| iscore | 0.000 | 0.000 | 0.000 | 0.051 | 0.000 | 0.000 | 0.000 | 0.000 | 0.053 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| Residue | C | W | K | M | T | G | D | E | S | D | L | G | R | P | K |
| Number | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 |
| NetOGlyc | gscore | 0.000 | 0.000 | 0.000 | 0.000 | 0.166 | 0.000 | 0.000 | 0.000 | 0.110 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| iscore | 0.000 | 0.000 | 0.000 | 0.000 | 0.035 | 0.000 | 0.000 | 0.000 | 0.024 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| Residue | L | T | Q | F | D | F | W | Y | C | L | H | G | C | L | M |
| Number | 76 | 77 | 78 | 79 | 80 | 81 | 82 | 83 | 84 | 85 | 86 | 87 | 88 | 89 | 90 |
| NetOGlyc | gscore | 0.000 | 0.160 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| iscore | 0.000 | 0.046 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| Residue | N | V | V | L | L | T | Q | V | V | A | G | A | R | I | W |
| Number | 91 | 92 | 93 | 94 | 95 | 96 | 97 | 98 | 99 | 100 | 101 | 102 | 103 | 104 | 105 |
| NetOGlyc | gscore | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.128 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| iscore | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.084 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| Residue | R | F | P | G | K | G | H | R | K | M | N | P | W | Y | L |
| Number | 106 | 107 | 108 | 109 | 110 | 111 | 112 | 113 | 114 | 115 | 116 | 117 | 118 | 119 | 120 |
| NetOGlyc | gscore | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| iscore | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| Residue | R | I | L | L | A | S | L | A | I | F | S | L | L | T | V |
| Number | 121 | 122 | 123 | 124 | 125 | 126 | 127 | 128 | 129 | 130 | 131 | 132 | 133 | 134 | 135 |
| NetOGlyc | gscore | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.077 | 0.000 | 0.000 | 0.000 | 0.000 | 0.063 | 0.000 | 0.000 | 0.102 | 0.000 |
| iscore | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.063 | 0.000 | 0.000 | 0.000 | 0.000 | 0.053 | 0.000 | 0.000 | 0.070 | 0.000 |
| Residue | Q | F | M | Y | S | N | Y | W | Y | D | W | H | N | S | R |
| Number | 136 | 137 | 138 | 139 | 140 | 141 | 142 | 143 | 144 | 145 | 146 | 147 | 148 | 149 | 150 |
| NetOGlyc | gscore | 0.000 | 0.000 | 0.000 | 0.000 | 0.091 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.072 | 0.000 |
| iscore | 0.000 | 0.000 | 0.000 | 0.000 | 0.036 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.034 | 0.000 |
| Residue | T | L | A | Y | C | N | N | L | F | L | L | K | I | S | M |
| Number | 151 | 152 | 153 | 154 | 155 | 156 | 157 | 158 | 159 | 160 | 161 | 162 | 163 | 164 | 165 |
| NetOGlyc | gscore | 0.113 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.106 | 0.000 |
| iscore | 0.023 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.054 | 0.000 |
| Residue | S | L | I | K | Y | I | P | Q | V | T | H | N | S | T | R |
| Number | 166 | 167 | 168 | 169 | 170 | 171 | 172 | 173 | 174 | 175 | 176 | 177 | 178 | 179 | 180 |
| NetOGlyc | gscore | 0.097 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.240 | 0.000 | 0.000 | 0.204 | 0.275 | 0.000 |
| iscore | 0.090 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.081 | 0.000 | 0.000 | 0.020 | 0.028 | 0.000 |
| Residue | K | S | M | D | C | F | P | I | Q | G | V | F | L | D | V |
| Number | 181 | 182 | 183 | 184 | 185 | 186 | 187 | 188 | 189 | 190 | 191 | 192 | 193 | 194 | 195 |
| NetOGlyc | gscore | 0.000 | 0.181 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| iscore | 0.000 | 0.046 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| Residue | T | G | G | I | A | S | L | L | Q | L | I | W | Q | L | S |
| Number | 196 | 197 | 198 | 199 | 200 | 201 | 202 | 203 | 204 | 205 | 206 | 207 | 208 | 209 | 210 |
| NetOGlyc | gscore | 0.158 | 0.000 | 0.000 | 0.000 | 0.000 | 0.124 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.127 |
| iscore | 0.084 | 0.000 | 0.000 | 0.000 | 0.000 | 0.028 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.032 |
| Residue | N | D | Q | G | F | S | L | D | T | F | V | T | N | F | G |
| Number | 211 | 212 | 213 | 214 | 215 | 216 | 217 | 218 | 219 | 220 | 221 | 222 | 223 | 224 | 225 |
| NetOGlyc | gscore | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.117 | 0.000 | 0.000 | 0.194 | 0.000 | 0.000 | 0.182 | 0.000 | 0.000 | 0.000 |
| iscore | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.058 | 0.000 | 0.000 | 0.049 | 0.000 | 0.000 | 0.074 | 0.000 | 0.000 | 0.000 |
| Residue | K | V | G | L | S | M | V | T | L | I | F | N | F | I | F |
| Number | 226 | 227 | 228 | 229 | 230 | 231 | 232 | 233 | 234 | 235 | 236 | 237 | 238 | 239 | 240 |
| NetOGlyc | gscore | 0.000 | 0.000 | 0.000 | 0.000 | 0.082 | 0.000 | 0.000 | 0.116 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| iscore | 0.000 | 0.000 | 0.000 | 0.000 | 0.066 | 0.000 | 0.000 | 0.070 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| Residue | I | M | Q | W | F | V | Y | R | S | R | G | H | D | L | A |
| Number | 241 | 242 | 243 | 244 | 245 | 246 | 247 | 248 | 249 | 250 | 251 | 252 | 253 | 254 | 255 |
| NetOGlyc | gscore | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.114 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| iscore | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.077 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| Residue | S | E | Y | P | L |
| Number | 256 | 257 | 258 | 259 | 260 |
| NetOGlyc | gscore | 0.288 | 0.000 | 0.000 | 0.000 | 0.000 |
| iscore | 0.108 | 0.000 | 0.000 | 0.000 | 0.000 |
Threshold description :
G-scores greaterthan 0.5 are predicted as positive predictions. However for threonines an additional score is used: if the G-score is less than 0.5 but the I-score greater than 0.5 and there are no predicted neighbouring sites (distance less than 10 residues) the residue is also predicted as glycosylated.
Graphical Output :
Analysis results from program: PsiPred
Running PsiPred Version 2.4
Function : Secondary Structure Prediction
PSIPRED Local Server
| Residue | M | V | S | L | D | D | I | L | G | I | V | Y | V | T | S |
| Number | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 |
| PsiPred | ssscore | 9 | 0 | 5 | 7 | 8 | 9 | 9 | 9 | 9 | 9 | 9 | 9 | 9 | 9 | 9 |
| sspred | C | C | H | H | H | H | H | H | H | H | H | H | H | H | H |
| Residue | W | S | I | S | M | Y | P | P | I | I | T | N | W | R | H |
| Number | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 |
| PsiPred | ssscore | 9 | 9 | 9 | 8 | 5 | 1 | 0 | 5 | 6 | 6 | 6 | 3 | 0 | 1 | 0 |
| sspred | H | H | H | H | H | H | H | H | H | H | H | H | H | C | C |
| Residue | K | S | A | S | A | I | S | M | D | F | V | M | L | N | T |
| Number | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 |
| PsiPred | ssscore | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 8 | 9 | 9 | 9 | 9 | 9 | 9 | 9 |
| sspred | C | C | E | E | E | E | E | H | H | H | H | H | H | H | H |
| Residue | A | G | Y | S | Y | L | V | I | S | I | F | L | Q | L | Y |
| Number | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 |
| PsiPred | ssscore | 9 | 9 | 9 | 9 | 9 | 9 | 9 | 9 | 9 | 9 | 9 | 9 | 8 | 5 | 2 |
| sspred | H | H | H | H | H | H | H | H | H | H | H | H | H | H | H |
| Residue | C | W | K | M | T | G | D | E | S | D | L | G | R | P | K |
| Number | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 |
| PsiPred | ssscore | 0 | 4 | 5 | 6 | 5 | 4 | 3 | 2 | 0 | 1 | 3 | 4 | 5 | 5 | 3 |
| sspred | H | H | H | H | H | H | H | H | C | C | C | C | C | C | C |
| Residue | L | T | Q | F | D | F | W | Y | C | L | H | G | C | L | M |
| Number | 76 | 77 | 78 | 79 | 80 | 81 | 82 | 83 | 84 | 85 | 86 | 87 | 88 | 89 | 90 |
| PsiPred | ssscore | 0 | 4 | 8 | 8 | 9 | 9 | 9 | 9 | 9 | 9 | 9 | 9 | 9 | 9 | 9 |
| sspred | C | H | H | H | H | H | H | H | H | H | H | H | H | H | H |
| Residue | N | V | V | L | L | T | Q | V | V | A | G | A | R | I | W |
| Number | 91 | 92 | 93 | 94 | 95 | 96 | 97 | 98 | 99 | 100 | 101 | 102 | 103 | 104 | 105 |
| PsiPred | ssscore | 9 | 9 | 9 | 9 | 9 | 9 | 9 | 9 | 8 | 7 | 2 | 0 | 1 | 2 | 3 |
| sspred | H | H | H | H | H | H | H | H | H | H | H | C | C | C | C |
| Residue | R | F | P | G | K | G | H | R | K | M | N | P | W | Y | L |
| Number | 106 | 107 | 108 | 109 | 110 | 111 | 112 | 113 | 114 | 115 | 116 | 117 | 118 | 119 | 120 |
| PsiPred | ssscore | 5 | 6 | 9 | 9 | 9 | 9 | 9 | 8 | 8 | 6 | 7 | 8 | 9 | 9 | 9 |
| sspred | C | C | C | C | C | C | C | C | C | C | C | H | H | H | H |
| Residue | R | I | L | L | A | S | L | A | I | F | S | L | L | T | V |
| Number | 121 | 122 | 123 | 124 | 125 | 126 | 127 | 128 | 129 | 130 | 131 | 132 | 133 | 134 | 135 |
| PsiPred | ssscore | 9 | 9 | 9 | 9 | 8 | 8 | 8 | 9 | 9 | 8 | 8 | 8 | 6 | 5 | 6 |
| sspred | H | H | H | H | H | H | H | H | H | H | H | H | H | H | H |
| Residue | Q | F | M | Y | S | N | Y | W | Y | D | W | H | N | S | R |
| Number | 136 | 137 | 138 | 139 | 140 | 141 | 142 | 143 | 144 | 145 | 146 | 147 | 148 | 149 | 150 |
| PsiPred | ssscore | 5 | 5 | 3 | 0 | 1 | 1 | 0 | 0 | 0 | 2 | 1 | 2 | 3 | 2 | 2 |
| sspred | H | H | H | E | E | E | E | E | C | C | C | C | C | C | H |
| Residue | T | L | A | Y | C | N | N | L | F | L | L | K | I | S | M |
| Number | 151 | 152 | 153 | 154 | 155 | 156 | 157 | 158 | 159 | 160 | 161 | 162 | 163 | 164 | 165 |
| PsiPred | ssscore | 7 | 7 | 8 | 9 | 9 | 9 | 9 | 9 | 9 | 9 | 9 | 9 | 9 | 9 | 9 |
| sspred | H | H | H | H | H | H | H | H | H | H | H | H | H | H | H |
| Residue | S | L | I | K | Y | I | P | Q | V | T | H | N | S | T | R |
| Number | 166 | 167 | 168 | 169 | 170 | 171 | 172 | 173 | 174 | 175 | 176 | 177 | 178 | 179 | 180 |
| PsiPred | ssscore | 9 | 9 | 9 | 8 | 5 | 0 | 0 | 5 | 3 | 2 | 1 | 1 | 1 | 2 | 3 |
| sspred | H | H | H | H | H | H | H | H | H | H | H | C | C | C | C |
| Residue | K | S | M | D | C | F | P | I | Q | G | V | F | L | D | V |
| Number | 181 | 182 | 183 | 184 | 185 | 186 | 187 | 188 | 189 | 190 | 191 | 192 | 193 | 194 | 195 |
| PsiPred | ssscore | 4 | 4 | 1 | 1 | 0 | 4 | 6 | 9 | 9 | 9 | 9 | 9 | 9 | 9 | 8 |
| sspred | C | C | C | C | H | H | H | H | H | H | H | H | H | H | H |
| Residue | T | G | G | I | A | S | L | L | Q | L | I | W | Q | L | S |
| Number | 196 | 197 | 198 | 199 | 200 | 201 | 202 | 203 | 204 | 205 | 206 | 207 | 208 | 209 | 210 |
| PsiPred | ssscore | 7 | 2 | 6 | 9 | 9 | 9 | 9 | 9 | 9 | 9 | 9 | 8 | 6 | 4 | 3 |
| sspred | H | H | H | H | H | H | H | H | H | H | H | H | H | H | H |
| Residue | N | D | Q | G | F | S | L | D | T | F | V | T | N | F | G |
| Number | 211 | 212 | 213 | 214 | 215 | 216 | 217 | 218 | 219 | 220 | 221 | 222 | 223 | 224 | 225 |
| PsiPred | ssscore | 1 | 1 | 2 | 5 | 5 | 3 | 3 | 3 | 3 | 1 | 0 | 1 | 1 | 5 | 8 |
| sspred | H | C | C | C | C | C | H | H | H | H | C | C | H | H | H |
| Residue | K | V | G | L | S | M | V | T | L | I | F | N | F | I | F |
| Number | 226 | 227 | 228 | 229 | 230 | 231 | 232 | 233 | 234 | 235 | 236 | 237 | 238 | 239 | 240 |
| PsiPred | ssscore | 8 | 9 | 9 | 9 | 9 | 9 | 9 | 9 | 9 | 9 | 9 | 9 | 9 | 9 | 9 |
| sspred | H | H | H | H | H | H | H | H | H | H | H | H | H | H | H |
| Residue | I | M | Q | W | F | V | Y | R | S | R | G | H | D | L | A |
| Number | 241 | 242 | 243 | 244 | 245 | 246 | 247 | 248 | 249 | 250 | 251 | 252 | 253 | 254 | 255 |
| PsiPred | ssscore | 9 | 9 | 9 | 9 | 9 | 8 | 8 | 3 | 3 | 7 | 8 | 8 | 7 | 2 | 0 |
| sspred | H | H | H | H | H | H | H | H | C | C | C | C | C | C | C |
| Residue | S | E | Y | P | L |
| Number | 256 | 257 | 258 | 259 | 260 |
| PsiPred | ssscore | 0 | 2 | 6 | 9 | 9 |
| sspred | C | C | C | C | C |
Threshold description :
No specific threshold exists. Instead a confidence level ranging from 0 to 9 is used for the predictions. Higher the value, higher is the confidence.
Graphical Output :
Analysis results from program: DAS-TM filter
Running DAS-TM filter
Function : Transmembrane protein predictions
| Residue | M | V | S | L | D | D | I | L | G | I | V | Y | V | T | S |
| Number | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 |
| DAS-TM filter | Score | 0.266 | 0.531 | 0.759 | 1.009 | 1.201 | 1.499 | 1.875 | 2.193 | 2.447 | 2.700 | 2.860 | 3.001 | 3.147 | 2.947 | 2.755 |
| Residue | W | S | I | S | M | Y | P | P | I | I | T | N | W | R | H |
| Number | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 |
| DAS-TM filter | Score | 2.625 | 2.461 | 2.357 | 2.116 | 1.871 | 1.647 | 1.445 | 1.291 | 1.230 | 1.119 | 0.942 | 0.793 | 0.707 | 0.631 | 0.620 |
| Residue | K | S | A | S | A | I | S | M | D | F | V | M | L | N | T |
| Number | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 |
| DAS-TM filter | Score | 0.669 | 0.788 | 0.933 | 1.086 | 1.250 | 1.410 | 1.487 | 1.587 | 1.705 | 1.939 | 2.145 | 2.264 | 2.379 | 2.402 | 2.565 |
| Residue | A | G | Y | S | Y | L | V | I | S | I | F | L | Q | L | Y |
| Number | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 |
| DAS-TM filter | Score | 2.699 | 2.898 | 3.170 | 3.439 | 3.788 | 4.051 | 4.193 | 4.171 | 4.044 | 3.952 | 3.726 | 3.444 | 3.013 | 2.708 | 2.323 |
| Residue | C | W | K | M | T | G | D | E | S | D | L | G | R | P | K |
| Number | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 |
| DAS-TM filter | Score | 1.911 | 1.520 | 1.145 | 0.897 | 0.691 | 0.534 | 0.433 | 0.373 | 0.375 | 0.387 | 0.427 | 0.427 | 0.454 | 0.502 | 0.568 |
| Residue | L | T | Q | F | D | F | W | Y | C | L | H | G | C | L | M |
| Number | 76 | 77 | 78 | 79 | 80 | 81 | 82 | 83 | 84 | 85 | 86 | 87 | 88 | 89 | 90 |
| DAS-TM filter | Score | 0.676 | 0.763 | 0.862 | 1.000 | 1.120 | 1.347 | 1.555 | 1.820 | 2.070 | 2.293 | 2.445 | 2.674 | 2.950 | 3.200 | 3.352 |
| Residue | N | V | V | L | L | T | Q | V | V | A | G | A | R | I | W |
| Number | 91 | 92 | 93 | 94 | 95 | 96 | 97 | 98 | 99 | 100 | 101 | 102 | 103 | 104 | 105 |
| DAS-TM filter | Score | 3.428 | 3.628 | 3.744 | 3.686 | 3.540 | 3.249 | 2.971 | 2.806 | 2.586 | 2.248 | 1.909 | 1.613 | 1.372 | 1.218 | 0.965 |
| Residue | R | F | P | G | K | G | H | R | K | M | N | P | W | Y | L |
| Number | 106 | 107 | 108 | 109 | 110 | 111 | 112 | 113 | 114 | 115 | 116 | 117 | 118 | 119 | 120 |
| DAS-TM filter | Score | 0.751 | 0.623 | 0.494 | 0.419 | 0.356 | 0.324 | 0.337 | 0.386 | 0.479 | 0.671 | 0.864 | 1.168 | 1.578 | 1.992 | 2.448 |
| Residue | R | I | L | L | A | S | L | A | I | F | S | L | L | T | V |
| Number | 121 | 122 | 123 | 124 | 125 | 126 | 127 | 128 | 129 | 130 | 131 | 132 | 133 | 134 | 135 |
| DAS-TM filter | Score | 2.883 | 3.441 | 3.959 | 4.305 | 4.553 | 4.802 | 5.075 | 5.157 | 5.205 | 5.076 | 4.818 | 4.609 | 4.248 | 3.806 | 3.323 |
| Residue | Q | F | M | Y | S | N | Y | W | Y | D | W | H | N | S | R |
| Number | 136 | 137 | 138 | 139 | 140 | 141 | 142 | 143 | 144 | 145 | 146 | 147 | 148 | 149 | 150 |
| DAS-TM filter | Score | 2.799 | 2.466 | 2.113 | 1.752 | 1.385 | 1.109 | 0.927 | 0.776 | 0.646 | 0.509 | 0.462 | 0.445 | 0.471 | 0.587 | 0.687 |
| Residue | T | L | A | Y | C | N | N | L | F | L | L | K | I | S | M |
| Number | 151 | 152 | 153 | 154 | 155 | 156 | 157 | 158 | 159 | 160 | 161 | 162 | 163 | 164 | 165 |
| DAS-TM filter | Score | 0.885 | 1.087 | 1.235 | 1.379 | 1.533 | 1.726 | 1.961 | 2.276 | 2.536 | 2.703 | 2.730 | 2.649 | 2.681 | 2.568 | 2.470 |
| Residue | S | L | I | K | Y | I | P | Q | V | T | H | N | S | T | R |
| Number | 166 | 167 | 168 | 169 | 170 | 171 | 172 | 173 | 174 | 175 | 176 | 177 | 178 | 179 | 180 |
| DAS-TM filter | Score | 2.374 | 2.302 | 2.129 | 1.868 | 1.669 | 1.431 | 1.161 | 0.963 | 0.864 | 0.741 | 0.601 | 0.507 | 0.451 | 0.415 | 0.389 |
| Residue | K | S | M | D | C | F | P | I | Q | G | V | F | L | D | V |
| Number | 181 | 182 | 183 | 184 | 185 | 186 | 187 | 188 | 189 | 190 | 191 | 192 | 193 | 194 | 195 |
| DAS-TM filter | Score | 0.437 | 0.526 | 0.648 | 0.755 | 0.917 | 1.075 | 1.218 | 1.408 | 1.523 | 1.739 | 2.027 | 2.201 | 2.342 | 2.413 | 2.572 |
| Residue | T | G | G | I | A | S | L | L | Q | L | I | W | Q | L | S |
| Number | 196 | 197 | 198 | 199 | 200 | 201 | 202 | 203 | 204 | 205 | 206 | 207 | 208 | 209 | 210 |
| DAS-TM filter | Score | 2.599 | 2.673 | 2.760 | 2.857 | 2.834 | 2.763 | 2.733 | 2.578 | 2.313 | 2.198 | 2.004 | 1.762 | 1.491 | 1.363 | 1.162 |
| Residue | N | D | Q | G | F | S | L | D | T | F | V | T | N | F | G |
| Number | 211 | 212 | 213 | 214 | 215 | 216 | 217 | 218 | 219 | 220 | 221 | 222 | 223 | 224 | 225 |
| DAS-TM filter | Score | 0.971 | 0.860 | 0.856 | 0.916 | 1.017 | 1.083 | 1.199 | 1.226 | 1.336 | 1.487 | 1.577 | 1.619 | 1.717 | 1.935 | 2.042 |
| Residue | K | V | G | L | S | M | V | T | L | I | F | N | F | I | F |
| Number | 226 | 227 | 228 | 229 | 230 | 231 | 232 | 233 | 234 | 235 | 236 | 237 | 238 | 239 | 240 |
| DAS-TM filter | Score | 2.201 | 2.531 | 2.815 | 3.233 | 3.559 | 3.914 | 4.209 | 4.424 | 4.713 | 4.899 | 4.896 | 4.736 | 4.701 | 4.541 | 4.184 |
| Residue | I | M | Q | W | F | V | Y | R | S | R | G | H | D | L | A |
| Number | 241 | 242 | 243 | 244 | 245 | 246 | 247 | 248 | 249 | 250 | 251 | 252 | 253 | 254 | 255 |
| DAS-TM filter | Score | 3.771 | 3.253 | 2.775 | 2.432 | 2.108 | 1.775 | 1.386 | 1.049 | 0.813 | 0.634 | 0.488 | 0.389 | 0.326 | 0.291 | 0.247 |
| Residue | S | E | Y | P | L |
| Number | 256 | 257 | 258 | 259 | 260 |
| DAS-TM filter | Score | 0.201 | 0.147 | 0.107 | 0.069 | 0.043 |
Threshold description :
Emperical cuttoff value is 2.5.The residues for which the Scores are higher than both the preceding and following residues are considered as TM helix predictions
Graphical Output :
| Residue range | Domain Name | Annotation types ............ |
|---|
| 10 - 16 | TM Helix | Peak : 13
| Score : 3.147
| E-value : 5.620e-03
| Comment : !!! Warning! Potential signal peptide.
|
| 45 - 59 | TM Helix | Peak : 52
| Score : 4.193
| E-value : 1.400e-04
|
| 87 - 99 | TM Helix | Peak : 93
| Score : 3.744
| E-value : 6.835e-04
|
| 121 - 136 | TM Helix | Peak : 129
| Score : 5.205
| E-value : 3.934e-06
|
| 159 - 164 | TM Helix | Peak : 161
| Score : 2.730
| E-value : 2.444e-02
|
| 195 - 203 | TM Helix | Peak : 199
| Score : 2.857
| E-value : 1.562e-02
|
| 227 - 243 | TM Helix | Peak : 235
| Score : 4.899
| E-value : 1.159e-05
|
Q-Score(Quality) :0.97
Q-score is quality score for an entry of the query against a library of known TM-proteins and is used to judge whether the entry is a TM-protein or not.The value of the score is a real number between 0 and 1 (the higher the better)
Analysis results from program: TargetP
Running TargetP Version 1.01
Function : Protein subcellular location Prediction
mTP-pred : 0.068
Mitochondrial targeting peptide (mTP) prediction score
SP-pred : 0.449
Secretory pathway signal peptide (SP) prediction score
other-pred : 0.469
Other targeting peptide predictions score
Loc-pred :ANY OTHER LOCATION.
SUBCELLULAR LOCATION PREDICTION
RC-pred : 5
Reliability class(RC) is a measure of the size of the difference(diff) between the highest(winning) and the second highest output scores. It ranges from 1 to 5 and the lower the value of RC, the better the prediction.
RC 1: difference greater than 0.800
RC 2: difference less than 0.800 and greater than 0.600
RC 3: difference less than 0.600 and greater than 0.400
RC 4: difference less than 0.400 and greater than 0.200
RC 5: difference less than 0.200
TPlen-pred : no prediction
Target peptide cleavage site prediction says about the predicted length of the presequence(if any was predicted). The actual cleavage site prediction is performed by SignalP for SPs, and by ChloroP for cTPs. The mTP cleavage site prediction, however, is a TargetP-unique feature.
Analysis results from program: PrositeScan
Running PrositeScan Version 1.20
Function : Protein profiles and motifs Prediction
Local Prositescan Server
| Residue range | Domain Name | Annotation types ............ |
|---|
| 177 - 180 | ASN_GLYCOSYLATION | Pattern : PS00001
| Match : NSTR
|
| 178 - 180 | PKC_PHOSPHO_SITE | Pattern : PS00005
| Match : StR
|
| 179 - 181 | PKC_PHOSPHO_SITE | Pattern : PS00005
| Match : TrK
|
| 3 - 6 | CK2_PHOSPHO_SITE | Pattern : PS00006
| Match : SldD
|
| 65 - 68 | CK2_PHOSPHO_SITE | Pattern : PS00006
| Match : TgdE
|
| 77 - 80 | CK2_PHOSPHO_SITE | Pattern : PS00006
| Match : TqfD
|
| 87 - 92 | MYRISTYL | Pattern : PS00008
| Match : GClmNV
|
| 197 - 202 | MYRISTYL | Pattern : PS00008
| Match : GGiaSL
|
Analysis results from program: TMHMM
Running TMHMM Version 2.0
Function : predictions of transmembrane helices in protein
| Residue | M | V | S | L | D | D | I | L | G | I | V | Y | V | T | S |
| Number | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 |
| TMHMM | inscore | 0.585 | 0.584 | 0.584 | 0.584 | 0.584 | 0.584 | 0.027 | 0.027 | 0.023 | 0.023 | 0.023 | 0.023 | 0.023 | 0.023 | 0.023 |
| memscore | 0.000 | 0.000 | 0.000 | 0.004 | 0.022 | 0.119 | 0.719 | 0.719 | 0.730 | 0.957 | 0.961 | 0.961 | 0.962 | 0.962 | 0.962 |
| outscore | 0.414 | 0.414 | 0.414 | 0.410 | 0.393 | 0.295 | 0.253 | 0.253 | 0.245 | 0.018 | 0.014 | 0.014 | 0.014 | 0.014 | 0.014 |
| Residue | W | S | I | S | M | Y | P | P | I | I | T | N | W | R | H |
| Number | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 |
| TMHMM | inscore | 0.023 | 0.023 | 0.023 | 0.023 | 0.023 | 0.024 | 0.025 | 0.025 | 0.025 | 0.027 | 0.029 | 0.031 | 0.136 | 0.409 | 0.423 |
| memscore | 0.962 | 0.962 | 0.962 | 0.962 | 0.961 | 0.961 | 0.960 | 0.959 | 0.959 | 0.948 | 0.507 | 0.435 | 0.324 | 0.043 | 0.003 |
| outscore | 0.014 | 0.014 | 0.014 | 0.014 | 0.014 | 0.014 | 0.014 | 0.014 | 0.015 | 0.023 | 0.462 | 0.533 | 0.538 | 0.547 | 0.573 |
| Residue | K | S | A | S | A | I | S | M | D | F | V | M | L | N | T |
| Number | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 |
| TMHMM | inscore | 0.423 | 0.423 | 0.423 | 0.422 | 0.418 | 0.417 | 0.416 | 0.415 | 0.414 | 0.020 | 0.005 | 0.003 | 0.002 | 0.002 | 0.001 |
| memscore | 0.000 | 0.000 | 0.001 | 0.003 | 0.038 | 0.046 | 0.048 | 0.057 | 0.074 | 0.901 | 0.975 | 0.979 | 0.983 | 0.987 | 0.994 |
| outscore | 0.575 | 0.575 | 0.575 | 0.573 | 0.542 | 0.535 | 0.534 | 0.526 | 0.510 | 0.077 | 0.019 | 0.016 | 0.013 | 0.010 | 0.003 |
| Residue | A | G | Y | S | Y | L | V | I | S | I | F | L | Q | L | Y |
| Number | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 |
| TMHMM | inscore | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.001 | 0.002 | 0.005 | 0.045 | 0.049 | 0.056 |
| memscore | 0.997 | 0.998 | 0.998 | 0.998 | 0.998 | 0.998 | 0.998 | 0.998 | 0.998 | 0.997 | 0.995 | 0.991 | 0.939 | 0.932 | 0.896 |
| outscore | 0.001 | 0.001 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.001 | 0.001 | 0.001 | 0.002 | 0.014 | 0.017 | 0.046 |
| Residue | C | W | K | M | T | G | D | E | S | D | L | G | R | P | K |
| Number | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 |
| TMHMM | inscore | 0.102 | 0.138 | 0.565 | 0.573 | 0.574 | 0.574 | 0.575 | 0.575 | 0.575 | 0.575 | 0.575 | 0.575 | 0.575 | 0.575 | 0.575 |
| memscore | 0.837 | 0.794 | 0.013 | 0.003 | 0.001 | 0.001 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| outscore | 0.060 | 0.067 | 0.420 | 0.423 | 0.423 | 0.424 | 0.424 | 0.424 | 0.424 | 0.424 | 0.424 | 0.424 | 0.424 | 0.424 | 0.424 |
| Residue | L | T | Q | F | D | F | W | Y | C | L | H | G | C | L | M |
| Number | 76 | 77 | 78 | 79 | 80 | 81 | 82 | 83 | 84 | 85 | 86 | 87 | 88 | 89 | 90 |
| TMHMM | inscore | 0.574 | 0.574 | 0.574 | 0.563 | 0.559 | 0.442 | 0.244 | 0.035 | 0.033 | 0.014 | 0.014 | 0.013 | 0.011 | 0.011 | 0.010 |
| memscore | 0.001 | 0.018 | 0.019 | 0.060 | 0.067 | 0.191 | 0.702 | 0.944 | 0.949 | 0.973 | 0.977 | 0.981 | 0.986 | 0.987 | 0.988 |
| outscore | 0.423 | 0.406 | 0.406 | 0.375 | 0.373 | 0.366 | 0.053 | 0.020 | 0.017 | 0.011 | 0.008 | 0.005 | 0.002 | 0.001 | 0.000 |
| Residue | N | V | V | L | L | T | Q | V | V | A | G | A | R | I | W |
| Number | 91 | 92 | 93 | 94 | 95 | 96 | 97 | 98 | 99 | 100 | 101 | 102 | 103 | 104 | 105 |
| TMHMM | inscore | 0.010 | 0.010 | 0.010 | 0.010 | 0.011 | 0.012 | 0.017 | 0.018 | 0.023 | 0.040 | 0.044 | 0.107 | 0.119 | 0.120 | 0.341 |
| memscore | 0.988 | 0.988 | 0.988 | 0.988 | 0.988 | 0.986 | 0.981 | 0.980 | 0.969 | 0.943 | 0.909 | 0.819 | 0.747 | 0.665 | 0.303 |
| outscore | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.001 | 0.001 | 0.007 | 0.015 | 0.045 | 0.072 | 0.133 | 0.214 | 0.354 |
| Residue | R | F | P | G | K | G | H | R | K | M | N | P | W | Y | L |
| Number | 106 | 107 | 108 | 109 | 110 | 111 | 112 | 113 | 114 | 115 | 116 | 117 | 118 | 119 | 120 |
| TMHMM | inscore | 0.424 | 0.429 | 0.434 | 0.434 | 0.434 | 0.434 | 0.434 | 0.434 | 0.434 | 0.434 | 0.429 | 0.182 | 0.053 | 0.044 | 0.034 |
| memscore | 0.046 | 0.038 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.007 | 0.014 | 0.326 | 0.630 | 0.734 | 0.826 |
| outscore | 0.529 | 0.532 | 0.564 | 0.564 | 0.565 | 0.565 | 0.565 | 0.565 | 0.565 | 0.558 | 0.556 | 0.491 | 0.316 | 0.220 | 0.139 |
| Residue | R | I | L | L | A | S | L | A | I | F | S | L | L | T | V |
| Number | 121 | 122 | 123 | 124 | 125 | 126 | 127 | 128 | 129 | 130 | 131 | 132 | 133 | 134 | 135 |
| TMHMM | inscore | 0.030 | 0.003 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.001 | 0.005 |
| memscore | 0.852 | 0.983 | 0.989 | 0.996 | 0.999 | 0.999 | 0.999 | 0.999 | 0.999 | 0.999 | 0.999 | 0.999 | 0.999 | 0.998 | 0.992 |
| outscore | 0.116 | 0.013 | 0.010 | 0.003 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.002 |
| Residue | Q | F | M | Y | S | N | Y | W | Y | D | W | H | N | S | R |
| Number | 136 | 137 | 138 | 139 | 140 | 141 | 142 | 143 | 144 | 145 | 146 | 147 | 148 | 149 | 150 |
| TMHMM | inscore | 0.022 | 0.025 | 0.043 | 0.051 | 0.133 | 0.297 | 0.366 | 0.421 | 0.459 | 0.562 | 0.562 | 0.563 | 0.563 | 0.562 | 0.562 |
| memscore | 0.957 | 0.946 | 0.914 | 0.887 | 0.576 | 0.286 | 0.215 | 0.159 | 0.120 | 0.004 | 0.007 | 0.006 | 0.007 | 0.068 | 0.211 |
| outscore | 0.019 | 0.027 | 0.042 | 0.060 | 0.290 | 0.415 | 0.417 | 0.419 | 0.420 | 0.433 | 0.430 | 0.429 | 0.428 | 0.368 | 0.226 |
| Residue | T | L | A | Y | C | N | N | L | F | L | L | K | I | S | M |
| Number | 151 | 152 | 153 | 154 | 155 | 156 | 157 | 158 | 159 | 160 | 161 | 162 | 163 | 164 | 165 |
| TMHMM | inscore | 0.553 | 0.501 | 0.458 | 0.449 | 0.447 | 0.443 | 0.439 | 0.434 | 0.433 | 0.433 | 0.433 | 0.434 | 0.434 | 0.434 | 0.435 |
| memscore | 0.232 | 0.308 | 0.360 | 0.388 | 0.394 | 0.398 | 0.403 | 0.409 | 0.412 | 0.412 | 0.412 | 0.411 | 0.411 | 0.411 | 0.410 |
| outscore | 0.213 | 0.190 | 0.180 | 0.161 | 0.158 | 0.158 | 0.157 | 0.155 | 0.154 | 0.153 | 0.153 | 0.153 | 0.153 | 0.153 | 0.153 |
| Residue | S | L | I | K | Y | I | P | Q | V | T | H | N | S | T | R |
| Number | 166 | 167 | 168 | 169 | 170 | 171 | 172 | 173 | 174 | 175 | 176 | 177 | 178 | 179 | 180 |
| TMHMM | inscore | 0.436 | 0.439 | 0.445 | 0.483 | 0.486 | 0.492 | 0.538 | 0.688 | 0.698 | 0.709 | 0.710 | 0.712 | 0.713 | 0.714 | 0.714 |
| memscore | 0.409 | 0.406 | 0.400 | 0.357 | 0.353 | 0.341 | 0.277 | 0.121 | 0.103 | 0.057 | 0.011 | 0.003 | 0.001 | 0.000 | 0.000 |
| outscore | 0.154 | 0.154 | 0.154 | 0.159 | 0.159 | 0.165 | 0.184 | 0.189 | 0.197 | 0.233 | 0.277 | 0.284 | 0.284 | 0.285 | 0.285 |
| Residue | K | S | M | D | C | F | P | I | Q | G | V | F | L | D | V |
| Number | 181 | 182 | 183 | 184 | 185 | 186 | 187 | 188 | 189 | 190 | 191 | 192 | 193 | 194 | 195 |
| TMHMM | inscore | 0.714 | 0.714 | 0.713 | 0.709 | 0.328 | 0.259 | 0.223 | 0.153 | 0.140 | 0.064 | 0.040 | 0.036 | 0.036 | 0.036 | 0.036 |
| memscore | 0.000 | 0.000 | 0.001 | 0.006 | 0.488 | 0.600 | 0.648 | 0.755 | 0.785 | 0.881 | 0.910 | 0.915 | 0.916 | 0.916 | 0.916 |
| outscore | 0.285 | 0.285 | 0.284 | 0.283 | 0.182 | 0.139 | 0.128 | 0.090 | 0.073 | 0.054 | 0.049 | 0.047 | 0.047 | 0.047 | 0.047 |
| Residue | T | G | G | I | A | S | L | L | Q | L | I | W | Q | L | S |
| Number | 196 | 197 | 198 | 199 | 200 | 201 | 202 | 203 | 204 | 205 | 206 | 207 | 208 | 209 | 210 |
| TMHMM | inscore | 0.036 | 0.036 | 0.036 | 0.036 | 0.036 | 0.036 | 0.036 | 0.036 | 0.043 | 0.045 | 0.049 | 0.064 | 0.223 | 0.238 | 0.245 |
| memscore | 0.916 | 0.916 | 0.916 | 0.916 | 0.916 | 0.916 | 0.916 | 0.915 | 0.907 | 0.900 | 0.890 | 0.863 | 0.248 | 0.162 | 0.068 |
| outscore | 0.047 | 0.047 | 0.047 | 0.047 | 0.047 | 0.047 | 0.047 | 0.047 | 0.048 | 0.054 | 0.059 | 0.072 | 0.527 | 0.599 | 0.686 |
| Residue | N | D | Q | G | F | S | L | D | T | F | V | T | N | F | G |
| Number | 211 | 212 | 213 | 214 | 215 | 216 | 217 | 218 | 219 | 220 | 221 | 222 | 223 | 224 | 225 |
| TMHMM | inscore | 0.257 | 0.273 | 0.274 | 0.274 | 0.274 | 0.274 | 0.274 | 0.274 | 0.266 | 0.229 | 0.227 | 0.224 | 0.211 | 0.163 | 0.153 |
| memscore | 0.019 | 0.001 | 0.000 | 0.000 | 0.000 | 0.000 | 0.005 | 0.012 | 0.125 | 0.224 | 0.232 | 0.264 | 0.285 | 0.364 | 0.396 |
| outscore | 0.723 | 0.724 | 0.725 | 0.725 | 0.725 | 0.725 | 0.720 | 0.713 | 0.608 | 0.546 | 0.539 | 0.510 | 0.503 | 0.472 | 0.450 |
| Residue | K | V | G | L | S | M | V | T | L | I | F | N | F | I | F |
| Number | 226 | 227 | 228 | 229 | 230 | 231 | 232 | 233 | 234 | 235 | 236 | 237 | 238 | 239 | 240 |
| TMHMM | inscore | 0.149 | 0.051 | 0.006 | 0.001 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.001 | 0.006 |
| memscore | 0.402 | 0.869 | 0.961 | 0.988 | 0.997 | 0.999 | 0.999 | 0.999 | 0.999 | 0.999 | 0.999 | 0.999 | 0.999 | 0.998 | 0.992 |
| outscore | 0.447 | 0.079 | 0.032 | 0.009 | 0.001 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| Residue | I | M | Q | W | F | V | Y | R | S | R | G | H | D | L | A |
| Number | 241 | 242 | 243 | 244 | 245 | 246 | 247 | 248 | 249 | 250 | 251 | 252 | 253 | 254 | 255 |
| TMHMM | inscore | 0.029 | 0.172 | 0.219 | 0.221 | 0.233 | 0.255 | 0.457 | 0.633 | 0.662 | 0.723 | 0.724 | 0.725 | 0.725 | 0.725 | 0.725 |
| memscore | 0.968 | 0.816 | 0.735 | 0.732 | 0.713 | 0.670 | 0.346 | 0.102 | 0.068 | 0.002 | 0.001 | 0.000 | 0.000 | 0.000 | 0.000 |
| outscore | 0.001 | 0.010 | 0.044 | 0.046 | 0.053 | 0.073 | 0.195 | 0.264 | 0.268 | 0.273 | 0.274 | 0.274 | 0.274 | 0.274 | 0.274 |
| Residue | S | E | Y | P | L |
| Number | 256 | 257 | 258 | 259 | 260 |
| TMHMM | inscore | 0.725 | 0.725 | 0.725 | 0.725 | 0.725 |
| memscore | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| outscore | 0.274 | 0.274 | 0.274 | 0.274 | 0.274 |
Threshold description :
If memscore is the highest among the three, then it is shown as positive prediction.
Graphical Output :
| Residue range | Domain Name | Annotation types ............ |
|---|
| 1 - 6 | TM Helix | Orientation : inside
|
| 7 - 25 | TM Helix | Orientation : TMhelix
|
| 26 - 39 | TM Helix | Orientation : outside
|
| 40 - 62 | TM Helix | Orientation : TMhelix
|
| 63 - 82 | TM Helix | Orientation : inside
|
| 83 - 105 | TM Helix | Orientation : TMhelix
|
| 106 - 117 | TM Helix | Orientation : outside
|
| 118 - 140 | TM Helix | Orientation : TMhelix
|
| 141 - 184 | TM Helix | Orientation : inside
|
| 185 - 207 | TM Helix | Orientation : TMhelix
|
| 208 - 226 | TM Helix | Orientation : outside
|
| 227 - 246 | TM Helix | Orientation : TMhelix
|
| 247 - 260 | TM Helix | Orientation : inside
|
Number of predicted TMHs :6
It tells about the number of Transmembrane helices found for the query protein sequence
Exp number of AAs in TMHs : 136.43041
It tells about the expected number of amino acids in Transmembrane helices. If this number is larger than 18 it is very likely to be a transmembrane protein (OR have a signal peptide).
Exp number, first 60 AAs : 39.83526
It tells about the expected number of aminoacids in Transmembrane helices in the first 60 amino acids of the protein. If this number more than a few, you should be warned that a predicted transmembrane helix in the N-term could be a signal peptide.
Total prob of N-in :0.58508
It tells about the the total probability that the N-term is on the cytoplasmic side of the membrane.
Warning : POSSIBLE N-term signal sequence
It is a warning that is produced when Exp number, first 60 AAs is larger than 10.
Analysis results from program: Plasmit
Running Plasmit
Function : Prediction of mitochondrial transit peptides in Plasmodium falciparum
Jury :NON-MITOCHONDRIAL
Mitochondrial transit peptide prediction
Reliability score : 99%
Reliability score for the prediction
Analysis results from program: ChloroP
Running ChloroP Version 1.1
Function : prediction of the presence of chloroplast transit peptides (cTP) in protein sequences and the location of potential cTP cleavage sites
Score : 0.461
It is the output score from the second step network. The prediction cTP/no cTP is based solely on this score.
cTP :Non-Chloroplast Transit Peptide
It tells whether or not this is predicted as a cTP-containing sequence.
CS-score : 2.344
It is the MEME scoring matrix score for the suggested cleavage site.
cTPlength : 32
It is the predicted length of the presequence (Please note that the prediction of the transit peptide length is carried out and presented even if its presence is not predicted).
Analysis results from program: PSORT
Running PSORT Version old version
Function : prediction of protein localization sites
endoplasmic reticulum (membrane) :0.685(Affirmative)
Description not available
plasma membrane :0.640(Affirmative)
Description not available
Golgi body :0.460(Affirmative)
Description not available
endoplasmic reticulum (lumen) :0.100(Affirmative)
Description not available